Johnson injured his back in practice and after consulting with experts announced to the media his withdrawal from the tournament. Unlucky Johnson returned earlier this following knee surgery over the Christmas period.
"First I have to apologize to the fans and sponsors. With heartfelt regret, I won't be playing in the Masters due to a tweaked back," Johnson tweeted.
The five-time PGA Tour winner has played in the Masters three times, finishing tied for 30th in 2009 and tied for 38th the past two years.